8TF5

Crystal structure of orphan G protein-coupled receptor 6, pseudoapo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	123
Detector technology	PIXEL
Collection date	2020-02-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	57.033, 96.867, 97.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.440 - 2.100
R-factor	0.1861
Rwork	0.184
R-free	0.21410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.695
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.191	0.638
Number of reflections	31292	2492
<I/σ(I)>	9.2	1.2
Completeness [%]	96.8
Redundancy	6.9
CC(1/2)	0.930	0.677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	NaCH3COO, PEG 400, NaCl, PPG P40