8TE4
Crystal structure of the methyltransferase domain of R882H/N879A DNMT3A homotetramer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-05-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97648 |
Spacegroup name | P 3 |
Unit cell lengths | 178.068, 178.068, 109.909 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.760 - 2.650 |
R-factor | 0.2033 |
Rwork | 0.203 |
R-free | 0.23650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yx2 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.740 |
High resolution limit [Å] | 2.650 | 5.710 | 2.650 |
Rmerge | 0.155 | 0.038 | 1.562 |
Rmeas | 0.172 | 0.041 | 1.734 |
Rpim | 0.073 | 0.017 | 0.750 |
Total number of observations | 621954 | ||
Number of reflections | 113612 | 11387 | 11348 |
<I/σ(I)> | 7 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 5.5 | 5.7 | 5.3 |
CC(1/2) | 0.999 | 0.345 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2-0.4 M potassium sodium tartrate |