Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE MFX |
| Synchrotron site | SLAC LCLS |
| Beamline | MFX |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2018-12-12 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 1.3045 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 78.616, 78.616, 265.275 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.770 - 1.650 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5wp2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.918 |
| Data reduction software | cctbx.xfel |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.770 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 59457 | 2912 |
| <I/σ(I)> | 4.54 | 0.538 |
| Completeness [%] | 99.9 | 99.66 |
| Redundancy | 9.8 | 9.85 |
| CC(1/2) | 0.984 | 0.140 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2 M ammonium sulfate, 100 mM MES buffer pH 5.3 |
