8T9Z

Structural of M8C10 Fab in complex human metapneumovirus fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	I 2 2 2
Unit cell lengths	98.900, 117.670, 181.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.345 - 2.995
R-factor	0.2455
Rwork	0.243
R-free	0.28030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.741
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.350	3.130
High resolution limit [Å]	2.990	2.990
Rmerge	0.161
Rmeas	0.178
Rpim	0.073
Number of reflections	21582	2511
<I/σ(I)>	8.3
Completeness [%]	99.5
Redundancy	6
CC(1/2)	0.994	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20 % PEG 3350, 200 mM Sodium Iodide