8T7G
Structure of the CK variant of Fab F1 (FabC-F1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.707, 73.752, 70.029 |
| Unit cell angles | 90.00, 116.54, 90.00 |
Refinement procedure
| Resolution | 62.650 - 2.000 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8t7f |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.857 |
| Data reduction software | XDS (AUTOPROC) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.650 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.105 | 1.179 |
| Rmeas | 0.120 | |
| Rpim | 0.058 | 0.870 |
| Number of reflections | 40653 | 1987 |
| <I/σ(I)> | 12.7 | 1.1 |
| Completeness [%] | 96.4 | 78.1 |
| Redundancy | 3.8 | 2.7 |
| CC(1/2) | 0.996 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 22% PEG8000, 200 mM sodium chloride, 100 mM sodium acetate, pH 4.0 |
