8T70
Cryptococcus neoformans protein farnesyltransferase in complex with FPTII and TKCMIIM peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 140.768, 140.768, 129.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.770 - 1.892 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.21180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.789 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.770 | 1.959 |
| High resolution limit [Å] | 1.892 | 1.892 |
| Rmerge | 0.117 | 0.688 |
| Rmeas | 0.129 | 0.755 |
| Rpim | 0.052 | 0.306 |
| Number of reflections | 103014 | 10141 |
| <I/σ(I)> | 8.08 | 1.82 |
| Completeness [%] | 99.3 | 98.87 |
| Redundancy | 5.7 | 5.9 |
| CC(1/2) | 0.992 | 0.931 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 290 | 100mM CAPSO pH9.5, 50-75mM Li2SO4, 200mM NaCl, 16%-21% PEG4000. |
