8T4Z

T-cell receptor and lipid complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-09-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9536
Spacegroup name	P 21 21 21
Unit cell lengths	57.906, 79.036, 242.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.087 - 2.691
R-factor	0.1966
Rwork	0.193
R-free	0.26540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.172
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.470	2.800
High resolution limit [Å]	2.690	2.700
Rpim	0.040	0.332
Number of reflections	31771	4050
<I/σ(I)>	12.5
Completeness [%]	99.6
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	18-20% PEG3350, 8% Tacsimate pH 5.0