8T4R
RAG2-PHD finger in complex with H3K4tBuNle peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2022-03-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 46.499, 46.499, 79.896 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.270 - 1.200 |
R-factor | 0.1406 |
Rwork | 0.140 |
R-free | 0.16020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.153 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.270 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 31462 | 2806 |
<I/σ(I)> | 31.24 | 0.53 |
Completeness [%] | 98.4 | 86 |
Redundancy | 29.4 | |
CC(1/2) | 1.000 | 0.929 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% PEG 5000 monomethyl ether, 0.1 M Bis-Tris, pH 6.5 |