8T35
Crystal structure of K51 acetylated LC3A in complex with the LIR of TP53INP2/DOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97680 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.356, 49.927, 69.784 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 37.235 - 1.902 |
| R-factor | 0.2292 |
| Rwork | 0.228 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1-2575_1496: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.235 | 1.995 |
| High resolution limit [Å] | 1.902 | 1.926 |
| Number of reflections | 19838 | 1751 |
| <I/σ(I)> | 8.26 | |
| Completeness [%] | 98.3 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.997 | 0.693 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG4000, 0.1M Na-citrate pH 5.5 |
