8T31

Crystal structure of GABARAP in complex with the LIR of TP53INP2/DOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97750
Spacegroup name	P 21 21 21
Unit cell lengths	51.898, 95.783, 138.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.253 - 2.096
R-factor	0.2005
Rwork	0.197
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.253	2.171
High resolution limit [Å]	2.096	2.096
Number of reflections	37232	1455
<I/σ(I)>	10.78
Completeness [%]	90.0
Redundancy	5.2
CC(1/2)	0.997	0.222

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG3350, 0.1M Na-citrate, 5% isopropanol