8T2M
Crystal structure of GABARAP in complex with the LIR of NSs4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.18057 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.741, 59.119, 67.346 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.450 - 1.270 |
| R-factor | 0.1597 |
| Rwork | 0.159 |
| R-free | 0.17760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.964 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.450 | 1.310 |
| High resolution limit [Å] | 1.270 | 1.271 |
| Number of reflections | 33702 | 2064 |
| <I/σ(I)> | 20.69 | |
| Completeness [%] | 95.7 | |
| Redundancy | 11.3 | |
| CC(1/2) | 1.000 | 0.567 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate:HCl, pH 4.6, 8% PEG 4000 |
