8T2L
Crystal structure of LC3A in complex with the LIR of NSs4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97680 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.995, 50.267, 137.874 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.600 - 2.240 |
R-factor | 0.2354 |
Rwork | 0.231 |
R-free | 0.27620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.527 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.600 | 2.320 |
High resolution limit [Å] | 2.240 | 2.240 |
Number of reflections | 12899 | 1072 |
<I/σ(I)> | 10.9 | |
Completeness [%] | 93.3 | |
Redundancy | 6.2 | |
CC(1/2) | 0.997 | 0.747 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350, 0.2M Na-citrate, 5% Isopropanol, 0.1M MES buffer PH 6.5 |