8T1G
The crystal structure of hemagglutinin form a h7n9 influenza virus (a/shanghai/1/2013) in complex with antibody 1E11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-03-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 303.277, 303.277, 153.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.770 - 3.500 |
| R-factor | 0.1824 |
| Rwork | 0.182 |
| R-free | 0.21520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.657 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.560 |
| High resolution limit [Å] | 3.500 | 9.480 | 3.500 |
| Rmerge | 0.183 | 0.051 | 1.641 |
| Rmeas | 0.194 | 0.054 | 1.735 |
| Rpim | 0.062 | 0.018 | 0.556 |
| Total number of observations | 868574 | ||
| Number of reflections | 89938 | 4805 | 4457 |
| <I/σ(I)> | 3.3 | ||
| Completeness [%] | 99.9 | 99.4 | 100 |
| Redundancy | 9.7 | 8.6 | 9.6 |
| CC(1/2) | 0.992 | 0.998 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES (pH 7.5), 1.08 M sodium citrate |
