8T11

Crystal structure of the PEG10 promoter-bound ONECUT2 R479A/R480A mutant DNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2022-12-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	96.140, 78.154, 39.501
Unit cell angles	90.00, 111.44, 90.00

Refinement procedure

Resolution	29.430 - 2.910
R-factor	0.2546
Rwork	0.251
R-free	0.30770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	HKL-2000 (v721.3)
Data scaling software	HKL-2000 (v721.3)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.900	7.860	2.900
Rmerge	0.160	0.061	1.483
Rmeas	0.189	0.074	1.733
Rpim	0.099	0.042	0.890
Number of reflections	5971	297	291
<I/σ(I)>	7.4
Completeness [%]	99.0	95.5	99.7
Redundancy	3.6	3.2	3.7
CC(1/2)	0.990	0.992	0.584

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	10 % PEG 1K and 7.5 % PEG 8K