8SXU
Structure of LINE-1 ORF2p with an oligo(A) template
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM CPC |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.66 |
| Number of Particle | 1536512 |
| Software | cryoSPARC,RELION,PHENIX,Leginon,CTFFIND |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING ONLY |
