8SVA

Structure of the Rhodococcus sp. USK13 DarR-20 bp DNA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.989
Spacegroup name	P 65
Unit cell lengths	128.894, 128.894, 113.929
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.190 - 2.960
R-factor	0.2424
Rwork	0.239
R-free	0.27610
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	0.936
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	AutoSol
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.190	3.030
High resolution limit [Å]	2.960	2.960
Rpim	0.064	1.575
Number of reflections	22523	1477
<I/σ(I)>	8.9	0.5
Completeness [%]	99.7
Redundancy	7.3
CC(1/2)	0.998	0.137

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	0.1 M MES pH 6.5, 30% PEG 8000 and 0.1 M Calcium acetate