8SUV
CHIP-TPR in complex with the C-terminus of CHIC2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2022-12-18 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000020 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.526, 82.356, 78.394 |
Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
Resolution | 46.530 - 1.630 |
R-factor | 0.2744 |
Rwork | 0.274 |
R-free | 0.29030 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.550 |
Data reduction software | XDS (0.95) |
Data scaling software | XSCALE |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.530 | 1.688 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.149 | 2.287 |
Rmeas | 0.161 | 2.465 |
Rpim | 0.061 | 0.911 |
Number of reflections | 71293 | 50246 |
<I/σ(I)> | 8.52 | 0.88 |
Completeness [%] | 96.4 | 94.59 |
Redundancy | 7 | 7.2 |
CC(1/2) | 0.998 | 0.652 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.05 M Magnesium sulfate heptahydrate, 0.1 M HEPES, 28 % v/v PEG Smear Medium |