8SUF

The complex of TOL-1 ectodomain bound to LAT-1 Lectin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	74.152, 316.755, 172.441
Unit cell angles	90.00, 90.16, 90.00

Refinement procedure

Resolution	74.150 - 4.000
R-factor	0.2908
Rwork	0.288
R-free	0.33770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	200.000	4.420
High resolution limit [Å]	4.000	4.000
Rmerge	0.112	0.727
Number of reflections	37159	2858
<I/σ(I)>	6.2	2.1
Completeness [%]	55.6	16.7
Redundancy	3.4
CC(1/2)	0.996	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	0.2 M Sodium Malonate, 0.1 M Bis-Tris Propane pH 7.5, 26% PEG 3350, 1.2% Myo-Inositol