8STM
Crystal structure of KRAS-G75A mutant, GDP-bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 3 |
Unit cell lengths | 84.930, 84.930, 87.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.830 - 2.000 |
R-factor | 0.1641 |
Rwork | 0.163 |
R-free | 0.19231 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 1.147 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.130 | 0.087 | 1.130 |
Rmeas | 0.144 | 0.097 | 1.257 |
Number of reflections | 47789 | 539 | 3530 |
<I/σ(I)> | 7.95 | ||
Completeness [%] | 99.9 | ||
Redundancy | 5.3 | ||
CC(1/2) | 0.990 | 0.991 | 0.567 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2.2 M Ammonium Sulfate |