8SSW
Crystal structure of DEAD-box RNA helicase DDX3X in complex with ADP at pre-unwound state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 |
Unit cell lengths | 68.611, 68.611, 213.878 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.750 - 2.400 |
R-factor | 0.208 |
Rwork | 0.207 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.515 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.127 | 0.061 | 1.205 |
Rpim | 0.067 | 0.030 | 0.636 |
Number of reflections | 42842 | 4412 | 4273 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 97.1 | 99.5 | 96.7 |
Redundancy | 4.1 | 4.7 | 4.2 |
CC(1/2) | 0.996 | 0.471 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG3350, Magnesium Chloride, etc. |