8SRM
Structure of human ULK1 complex core (2:2:2 stoichiometry) of the ATG13(450-517) mutant
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.46 |
| Number of Particle | 968884 |
| Software | Topaz,cryoSPARC,PHENIX,ISOLDE,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
