8SP7

LINE-1 retrotransposon endonuclease domain complex with tranexamic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	C 2 2 21
Unit cell lengths	54.222, 64.671, 132.358
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.050 - 1.830
R-factor	0.2216
Rwork	0.220
R-free	0.25630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.901
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_4895)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.050	1.870
High resolution limit [Å]	1.830	1.830
Number of reflections	20957	1271
<I/σ(I)>	13.9
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.998	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris acetate pH 6.0, 0.2 M lithium sulfate, 30% PEG 2000 monomethyl ether