8SOZ
Structure of the complex formed by human interleukin-2 and scFv 602
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-01-30 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.920 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.192, 36.419, 64.189 |
Unit cell angles | 90.00, 108.12, 90.00 |
Refinement procedure
Resolution | 32.600 - 1.640 |
Rwork | 0.219 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.148 |
Data reduction software | autoPROC |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.1_4122: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.000 | 1.670 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.066 | 1.500 |
Number of reflections | 37048 | 3555 |
<I/σ(I)> | 5.97 | 1.01 |
Completeness [%] | 99.4 | 96.9 |
Redundancy | 3.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 15-30% PEG 3350, 0.1-0.35 ammonium fluoride |