8SMH

Chimeric ETS-domain of murine PU.1 harboring the corresponding beta-strand 3 (S3) residues from murine Ets-1 in complex with d(AATAAGCGGAAGTGGG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 1 21 1
Unit cell lengths	42.944, 60.710, 45.066
Unit cell angles	90.00, 117.28, 90.00

Refinement procedure

Resolution	33.430 - 1.370
R-factor	0.1447
Rwork	0.143
R-free	0.17720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.430	1.419
High resolution limit [Å]	1.370	1.370
Rmerge	0.038	0.451
Rmeas	0.046	0.540
Rpim	0.025	0.295
Number of reflections	42457	4177
<I/σ(I)>	16.94	2.24
Completeness [%]	98.0	96.77
Redundancy	3.3	3.2
CC(1/2)	0.998	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Sodium Acetate, pH=4.6, 2% PEG 3350