8SJ9

Crystal structure of the H1 hemagglutinin COBRA X6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03
Spacegroup name	P 1 21 1
Unit cell lengths	68.504, 218.725, 68.572
Unit cell angles	90.00, 109.68, 90.00

Refinement procedure

Resolution	44.430 - 3.250
R-factor	0.2239
Rwork	0.221
R-free	0.27630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.452
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.450
High resolution limit [Å]	3.250	3.250
Rmerge	0.143	1.268
Number of reflections	29281	2921
<I/σ(I)>	6	1
Completeness [%]	98.1	98.35
Redundancy	3.5
CC(1/2)	0.995	0.312

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M Tris pH 8.4, 22% PEG 3350