8SIJ
Crystal structure of F. varium tryptophanase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 98.589, 98.589, 278.515 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.540 - 2.600 |
R-factor | 0.241 |
Rwork | 0.240 |
R-free | 0.26020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.184 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.540 | 2.695 |
High resolution limit [Å] | 2.600 | 2.602 |
Rmerge | 0.141 | 1.662 |
Number of reflections | 43050 | 4146 |
<I/σ(I)> | 9.3 | 0.81 |
Completeness [%] | 99.6 | 98.13 |
Redundancy | 8.2 | |
CC(1/2) | 0.997 | 0.590 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.15 M tris hydrochloride pH 7, 1 M sodium citrate, and 0.2 M sodium chloride. Crystals formed in a 2:1 protein (11.1 mg/mL) to mother liquor ratio |