8SHU

Structure of mouse cGAS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	I 1 2 1
Unit cell lengths	87.892, 61.054, 105.704
Unit cell angles	90.00, 94.57, 90.00

Refinement procedure

Resolution	28.760 - 1.710
R-factor	0.17899
Rwork	0.177
R-free	0.21139
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k8v
RMSD bond length	0.010
RMSD bond angle	1.642
Data reduction software	XDS (20210205)
Data scaling software	Aimless (0.7.7)
Phasing software	MOLREP (11.7.03)
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.760	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.039	0.437
Rmeas	0.055	0.618
Rpim	0.039	0.437
Total number of observations		5780
Number of reflections	58990	3022
<I/σ(I)>	10.5	1.7
Completeness [%]	97.8
Redundancy	1.9	1.9
CC(1/2)	0.998	0.730

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277.15	0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate trihydrate, pH 6.5, 20% w/v PEG8000