8SHJ
Crystal structure of the WD-repeat domain of human WDR91 in complex with MR45279
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-27 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.859, 121.374, 131.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.250 - 2.210 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.250 |
| High resolution limit [Å] | 2.210 | 6.000 | 2.210 |
| Rmerge | 0.099 | 0.042 | 0.992 |
| Rmeas | 0.106 | 0.046 | 1.068 |
| Rpim | 0.039 | 0.017 | 0.391 |
| Total number of observations | 463068 | ||
| Number of reflections | 62725 | 3374 | 3080 |
| <I/σ(I)> | 5.7 | ||
| Completeness [%] | 99.7 | 99.5 | 99.5 |
| Redundancy | 7.4 | 6.8 | 7.3 |
| CC(1/2) | 0.996 | 0.999 | 0.744 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 12% (w/v) PEG3350, 0.2 M KCl, 0.1 M MES pH 6.5 |
