8S8Q

Structure of the Interfilum paradoxum LFY DNA-binding domain bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8734
Spacegroup name	P 61
Unit cell lengths	144.890, 144.890, 69.836
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.430 - 2.950
R-factor	0.19587
Rwork	0.194
R-free	0.23693
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.714
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.430	47.300	2.790
High resolution limit [Å]	2.720	12.170	2.720
Rmerge	0.148	0.025	3.375
Rmeas	0.159	0.027	3.776
Number of reflections	22512	274	1588
<I/σ(I)>	9.26
Completeness [%]	99.5
Redundancy	7
CC(1/2)	0.999	1.000	0.095

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 8000 30%, 10mM magnesium acetate