8S4Y

Crystal structure of an APP-talin (F2F3) chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.95375
Spacegroup name	P 21 21 21
Unit cell lengths	60.388, 64.081, 65.191
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.700 - 2.700
R-factor	0.2167
Rwork	0.211
R-free	0.26720
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.032
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.700	2.740
High resolution limit [Å]	2.700	2.700
Rmerge	0.117	0.781
Rmeas	0.128	0.850
Rpim	0.050	0.330
Number of reflections	7374	359
<I/σ(I)>	12.8	1.9
Completeness [%]	100.0	97
Redundancy	6.5	6.6
CC(1/2)	0.993	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.1 M Tris pH 8.5, 10% v/v ethanol