8S31

Crystal structure of human PLK1 Polo-Box Domain in complex with Mis18BP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.67021
Spacegroup name	P 32 2 1
Unit cell lengths	67.147, 67.147, 254.536
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.080 - 2.134
R-factor	0.1967
Rwork	0.195
R-free	0.22440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.266
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.080	2.211
High resolution limit [Å]	2.134	2.134
Rmerge	0.104	0.723
Rmeas	0.110	0.766
Rpim		0.247
Number of reflections	37897	3442
<I/σ(I)>	15.79	2.85
Completeness [%]	99.2	92.13
Redundancy	10
CC(1/2)	0.999	0.799

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50 mM MES (pH 6.0), 20 mM Sodium Oxalate, 1.2 M Sodium Malonate