8S30
Crystal structure of human PLK1 Polo-Box Domain in complex with Mis18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.979499 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.138, 56.568, 49.576 |
Unit cell angles | 90.00, 115.74, 90.00 |
Refinement procedure
Resolution | 44.660 - 1.940 |
R-factor | 0.2194 |
Rwork | 0.217 |
R-free | 0.27070 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.097 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.660 | 2.009 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.888 | |
Number of reflections | 15410 | 1116 |
<I/σ(I)> | 7.16 | |
Completeness [%] | 94.6 | |
Redundancy | 3.2 | |
CC(1/2) | 0.978 | 0.271 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.09 M Halogen mix (NaF, NaBr, NaI), 0.1 M buffer system 2 (Sodium HEPES and MOPS) (pH7.5), 37% precipitant mix MPD_P1K_P3350 (MPD (racemic), PEG 1K, PEG 3350) |