8RWB

Crystal structure of ULBP6 in complex with a blocking antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-02
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9998
Spacegroup name	C 1 2 1
Unit cell lengths	106.551, 95.803, 97.203
Unit cell angles	90.00, 117.74, 90.00

Refinement procedure

Resolution	86.031 - 2.307
Rwork	0.216
R-free	0.28650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.422
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.031	2.658
High resolution limit [Å]	2.307	2.307
Number of reflections	19970	999
<I/σ(I)>	7.2
Completeness [%]	92.3
Redundancy	4
CC(1/2)	0.991	0.581

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Glycerol 10.0 %v/v MES: 0.10 M pH:6.00 PEG 1K 5.0 %w/v PEG 600 30.0 %w/v