8RVK
Maltodextrin phosphorylase (MalP) in complex with a alpha-1,2-cyclophellitol analogue
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 110.498, 119.790, 150.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.503 - 2.180 |
Rwork | 0.177 |
R-free | 0.22940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 2.381 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 71.503 | 1.920 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 160021 | 7599 |
<I/σ(I)> | 5.9 | 0.3 |
Completeness [%] | 99.9 | 97.1 |
Redundancy | 1.9 | |
CC(1/2) | 0.992 | 0.284 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2-0.5 M Li2SO4 monohydrate, 0.1 M Bis-Tris pH 5.5-7.0 and 25-30% (w/v) PEG 3350 |