8RUU

Fabs derived from bimekizumab in complex with IL-17F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	193
Detector technology	PIXEL
Collection date	2014-11-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	99.150, 141.750, 145.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.950 - 2.810
R-factor	0.206
Rwork	0.204
R-free	0.24150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jpy
RMSD bond length	0.007
RMSD bond angle	1.019
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.950	2.880
High resolution limit [Å]	2.810	2.810
Rmerge	0.048	0.580
Rmeas	0.056	0.680
Rpim	0.022	0.258
Number of reflections	50718	25323
<I/σ(I)>	23.2	2.9
Completeness [%]	99.9	99.9
Redundancy	6.5	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	PEG 8000, lithium sulphate, glycerol