8RUM
Structure of Oceanobacillus iheyensis group II intron in the presence of Li+, Mg2+, and intronistat B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-12-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.96 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.149, 94.834, 224.204 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.070 - 3.620 |
R-factor | 0.17601 |
Rwork | 0.172 |
R-free | 0.24169 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 2.116 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.070 | 3.748 |
High resolution limit [Å] | 3.600 | 3.600 |
Number of reflections | 22380 | 2183 |
<I/σ(I)> | 12.57 | |
Completeness [%] | 99.8 | |
Redundancy | 6.3 | |
CC(1/2) | 1.000 | 0.535 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 303.15 | 100 mM Mg-Acetate, 200 mM KCl, 50 mM LiCl, 50 mM Na-HEPES pH 7.0, 4% PEG 8000 |