8RU4
Crystal structure of Human Catenin Beta-1 in complex with stitched peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9212 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.567, 103.617, 187.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.961 - 2.130 |
| Rwork | 0.201 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.196 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.961 | 2.310 |
| High resolution limit [Å] | 2.129 | 2.129 |
| Rpim | 0.036 | 0.319 |
| Number of reflections | 51113 | 2551 |
| <I/σ(I)> | 11.9 | 1.3 |
| Completeness [%] | 94.7 | 64.9 |
| Redundancy | 13.7 | 14.2 |
| CC(1/2) | 0.999 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 100 mM TRIS pH 8.75, 2%(w/v) PEG-6000, cryoprotected in 30% (w/v) Glucose. |
