8RU3
Crystal structure of beta-catenin in complex with alpha-helical peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-29 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.526, 91.255, 112.984 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.991 - 2.001 |
| Rwork | 0.184 |
| R-free | 0.23170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.921 |
| Data reduction software | xia2.multiplex |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.013 | 2.207 |
| High resolution limit [Å] | 2.001 | 2.002 |
| Rpim | 0.030 | 0.124 |
| Number of reflections | 18536 | 926 |
| <I/σ(I)> | 11.6 | 1.2 |
| Completeness [%] | 93.5 | 61.8 |
| Redundancy | 52 | 30.9 |
| CC(1/2) | 1.000 | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 300 | Crystals grown in 100 mM NaKPO4, 2M NaCl. |
