8RTD
Stalk-Arches-IMC structure from the fully-assembled R388 type IV secretion system determined by cryo-EM.
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.33 |
Software | cryoSPARC,PHENIX |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |