8RS5
Crystal structure of Methanopyrus kandleri malate dehydrogenase mutant 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-11 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.360, 74.360, 267.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.950 |
| R-factor | 0.2593 |
| Rwork | 0.258 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.893 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 55448 | 4057 |
| <I/σ(I)> | 8.16 | |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 7.39 | 7.68 |
| CC(1/2) | 0.997 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M potassium citrate tribasic monohydrate, 13-16% PEG 3350 |
