8RRO
G12V-TCR complex with HLA-A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 2.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 131.477, 195.787, 442.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.460 - 3.500 |
| R-factor | 0.2402 |
| Rwork | 0.238 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 6.707 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.460 | 3.626 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 137380 | 11066 |
| <I/σ(I)> | 3.46 | 0.25 |
| Completeness [%] | 84.4 | |
| Redundancy | 1.1 | |
| CC(1/2) | 1.000 | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.1M Bis-Tris Propane (pH 6.5), 0.2M Na2SO4, 14-20% PEG 3350 |
