8RQA

Crystal structure of CRBN-midi in complex with Lenalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.6199
Spacegroup name	C 2 2 21
Unit cell lengths	51.989, 95.717, 148.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.540 - 2.500
R-factor	0.3019
Rwork	0.300
R-free	0.34200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.688
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.540	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.200	1.782
Number of reflections	13030	1181
<I/σ(I)>	5.046	1.17
Completeness [%]	99.7	96.4
Redundancy	4.8
CC(1/2)	0.990	0.420

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	0.1 M Tris pH 8, 20 % w/v PEG 4000, 1.2 % myo-inositol