8RQ8

Crystal structure of CRBN-midi in complex with mezigdomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00
Spacegroup name	P 43 21 2
Unit cell lengths	51.046, 51.046, 267.677
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.590 - 2.190
R-factor	0.2589
Rwork	0.258
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.328
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.900	2.370
High resolution limit [Å]	2.190	2.190
Number of reflections	13035	653
<I/σ(I)>	6.4
Completeness [%]	91.8
Redundancy	6.5
CC(1/2)	0.989	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	214 uM mezigdomide 0.2 M sodium acetate, 25% (w/v) PEG 3350, and 0.1 M BIS-TRIS pH 6.5