8RQ2
Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation III
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.44 |
| Software | Warp,cryoSPARC,RELION,Coot,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
