8ROE
Human cohesin SMC1A-HD(shortCC-EQ)/RAD21-C complex - ADP-bound conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-23 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.000002 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 66.038, 113.835, 134.186 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.490 - 1.360 |
R-factor | 0.2051 |
Rwork | 0.204 |
R-free | 0.21940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.833 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.440 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.067 | 2.855 |
Number of reflections | 108065 | 17268 |
<I/σ(I)> | 20.31 | 0.92 |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 13.2 | 13.5 |
CC(1/2) | 1.000 | 0.387 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium malonate; 0.1 M Bis Tris propane pH 6.5; 20 % w/v PEG 3350 |