8RO9

Human cohesin SMC1A-HD(longCC-EQ)/RAD21-C complex - Open/closed P-loop conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979950
Spacegroup name	C 1 2 1
Unit cell lengths	187.918, 64.597, 89.949
Unit cell angles	90.00, 99.74, 90.00

Refinement procedure

Resolution	44.646 - 1.767
R-factor	0.1911
Rwork	0.190
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.870
High resolution limit [Å]	1.767	1.767
Rmerge	0.055	1.277
Number of reflections	103953	16610
<I/σ(I)>	15.94	1.05
Completeness [%]	99.7	98.7
Redundancy	6.8	6.2
CC(1/2)	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M sodium bromide; 0.1 M Bis-Tris propane pH 6.5; 20% w/v PEG 3350