8RHQ
Crystal structure of HLA-A*11:01 in complex with SVLNDIFSRL, an 10-mer epitope from SARS-CoV-2 Spike (S975-984)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.953732 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.492, 85.492, 312.028 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.750 - 2.000 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.651 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21rc1_5127) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.720 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.147 | 0.596 |
| Rmeas | 0.151 | 0.612 |
| Rpim | 0.034 | 0.140 |
| Number of reflections | 90742 | 4424 |
| <I/σ(I)> | 15.1 | 5.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.8 | 19 |
| CC(1/2) | 0.997 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 0.2M Sodium acetate tihydrate pH 7.0 |
