8RHN
Structure of the 55LCC ATPase complex
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | TFS Selectris |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.5 |
| Number of Particle | 4182380 |
| Software | RELION,cryoSPARC,PHENIX,EPU,UCSF ChimeraX |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
