8RDT
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with spiro-isoxazol based compound 8j
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-28 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.441, 59.488, 88.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.300 - 1.950 |
R-factor | 0.20088 |
Rwork | 0.199 |
R-free | 0.24169 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.624 |
Data reduction software | XDS |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.303 | 49.303 | 2.070 |
High resolution limit [Å] | 1.950 | 5.810 | 1.950 |
Rmeas | 0.106 | 0.044 | 1.817 |
Number of reflections | 41768 | 1577 | 6715 |
<I/σ(I)> | 10.67 | 37.71 | 1.05 |
Completeness [%] | 99.9 | 99.9 | 99.6 |
Redundancy | 7.01 | 6.75 | 7.19 |
CC(1/2) | 0.999 | 0.999 | 0.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3 mM Thalidomide |