8RD2

Trypanosoma brucei Invariant Surface Glycoprotein 75 (ISG75)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.033
Spacegroup name	P 41
Unit cell lengths	120.010, 120.010, 107.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.670 - 2.690
R-factor	0.2308
Rwork	0.228
R-free	0.27210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.481
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.670	3.000
High resolution limit [Å]	2.690	2.700
Rmerge	0.078	1.004
Number of reflections	22111	1106
<I/σ(I)>	10.38
Completeness [%]	53.3	10.6
Redundancy	4.65
CC(1/2)	0.998	0.541

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	281	20% PEG3350 100 mM HEPES pH 7.5 20 mM Sorbitol